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Registro Completo |
Biblioteca(s): |
Embrapa Algodão; Embrapa Meio-Norte; Embrapa Semiárido; Embrapa Unidades Centrais. |
Data corrente: |
10/11/1999 |
Data da última atualização: |
19/08/2013 |
Autoria: |
SANTOS, R. F. dos; FREIRE, E. C.; CARVALHO, O. S. |
Afiliação: |
RAIMUNDO FRANCISCO DOS SANTOS, CPATSA; Embrapa Algodão. |
Título: |
Análise de alguns elos da cadeia produtiva do algodão no Brasil. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Campina Grande: EMBRAPA-CNPA, 1998. |
Páginas: |
4p. |
Série: |
(EMBRAPA-CNPA.Comunicado Tecnico,87) |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Cadeia agroindustrial; Chain; Crise do algodão; Production. |
Thesagro: |
Algodão; Análise Econômica; Cadeia Produtiva; Gossypium Hirsutum; Produção. |
Thesaurus Nal: |
cotton; Gossypium. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/59984/1/COMTEC87.pdf
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Marc: |
LEADER 00774nam a2200289 a 4500 001 1270895 005 2013-08-19 008 1998 bl uuuu u0uu1 u #d 100 1 $aSANTOS, R. F. dos 245 $aAnálise de alguns elos da cadeia produtiva do algodão no Brasil. 260 $aCampina Grande: EMBRAPA-CNPA$c1998 300 $a4p. 490 $a(EMBRAPA-CNPA.Comunicado Tecnico,87) 650 $acotton 650 $aGossypium 650 $aAlgodão 650 $aAnálise Econômica 650 $aCadeia Produtiva 650 $aGossypium Hirsutum 650 $aProdução 653 $aBrasil 653 $aCadeia agroindustrial 653 $aChain 653 $aCrise do algodão 653 $aProduction 700 1 $aFREIRE, E. C. 700 1 $aCARVALHO, O. S.
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Embrapa Algodão (CNPA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
31/03/1993 |
Data da última atualização: |
05/03/2024 |
Autoria: |
KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de; GOLDBACH, R. W.; PETERS, D. |
Afiliação: |
ANTONIO CARLOS DE AVILA, CNPH. |
Título: |
Immuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts. |
Ano de publicação: |
1992 |
Fonte/Imprenta: |
Journal of Virological Methods, v. 38, n. 3, p. 313-322, Aug./Sept. 1992. |
DOI: |
https://doi.org/10.1016/0166-0934(92)90076-P |
Idioma: |
Inglês |
Conteúdo: |
Tomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not. |
Palavras-Chave: |
Deteccao; Immunogold; Imunoflorescencia; Labelling; TSWV; Vira-Cabeca. |
Thesaurus NAL: |
detection; Tomato spotted wilt virus. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02285naa a2200277 a 4500 001 1749564 005 2024-03-05 008 1992 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/0166-0934(92)90076-P$2DOI 100 1 $aKITAJIMA, E. W. 245 $aImmuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts.$h[electronic resource] 260 $c1992 520 $aTomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not. 650 $adetection 650 $aTomato spotted wilt virus 653 $aDeteccao 653 $aImmunogold 653 $aImunoflorescencia 653 $aLabelling 653 $aTSWV 653 $aVira-Cabeca 700 1 $aRESENDE, R. de O. 700 1 $aAVILA, A. C. de 700 1 $aGOLDBACH, R. W. 700 1 $aPETERS, D. 773 $tJournal of Virological Methods$gv. 38, n. 3, p. 313-322, Aug./Sept. 1992.
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